GENERAL SUMMARY/ OVERVIEW STATEMENT:
FUNCTIONAL GENOMICS OF OBESITY AND DIABETES.
Postdoctoral fellow position available in the Soukas Laboratory at the Massachusetts General Hospital and Harvard Medical School, in Boston, Massachusetts. The Soukas Laboratory uses vertebrate and invertebrate model systems to study the genetic pathways that contribute to obesity and diabetes. A particular area of focus are the bodys defenses against starvation, and how conserved starvation defense pathways contribute to changes in body fat mass. The lab employs a multidisciplinary approach using genomics, genetics, molecular biology, biochemistry, and microscopy.
The Soukas Lab is part of the Department of Medicine and Diabetes Unit, and is located in the ultramodern, state-of-the-art Simches Research Building within the Center for Human Genetic Research at the Massachusetts General Hospital. The Soukas Laboratory has Associate Membership at the Broad Institute and has formal access to the myriad genomic, proteomic, and metabolomics resources of the Broad.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
The postdoctoral fellow will conduct systems level genetic and genomic experiments using vertebrate and invertebrate systems (mouse and C. elegans) to identify and analyze genes required for normal lipid and glucose homeostasis. This is a unique opportunity to leverage the strength of invertebrate genetics with the medical relevance of mammalian genetics to make discoveries that are important for human health and metabolic diseases. The fellow will be expected to conceive of and conduct hypothesis-driven research in conjunction with Dr. Soukas mentorship and guidance.
LICENSES, CERTIFICATIONS, and/or REGISTRATIONS:
SUPERVISORY RESPONSIBILITY (authority to hire, promote, or terminate):
The postdoctoral fellow will perform all work at the Massachusetts General Hospital within the Soukas Laboratory. Some work may be conducted in conjunction with specialized research groups at the Broad Institute, with which the Soukas Laboratory is formally associated.
SKILLS & COMPETENCIES REQUIRED:
Approaches which the postdoctoral fellow will use in the lab include:
* Engineering genetically modified mouse models of metabolism using CRISPR and lox-Cre recombination.
* murine physiology, energy expenditure analysis on Sable Promethion equipment, body composition, measurement of glucose and insulin tolerance, hyperinsulinemic euglycemic clamp study to define detailed insulin action in vivo
* Biochemical approaches to studying insulin signaling and molecular biological techniques to studying genome-wide expression profiles in metabolically important tissues,
* Primary and immortalized cell culture to model disease processes using RNAi and CRISPR-based gene targeting.
* GC/MS and LC/MS lipidomics and metabolomics
* Genome-wide RNAi screening for metabolic regulatory genes involved in lipid homeostasis and starvation survival, forward genetic screening for metabolic regulatory genes
* whole genome sequencing and expression profiling using next-generation sequencing
* Transgenic and genome engineering techniques
The successful applicant must have knowledge of and have practical knowledge of mouse and/or C. elegans genetics, basic knowledge of metabolic regulation, a strong history of productivity and primary literature publication.
First hand experience with physiologic, molecular genetic, and biochemical approaches to lipid and glucose homeostasis is not required but a distinct plus.
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.