Research Associate

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POST DATE 8/24/2016
END DATE 11/18/2016

Broad Institute Cambridge, MA

Cambridge, MA
AJE Ref #
Job Classification
Full Time
Job Type
Company Ref #
Entry Level (0 - 2 years)
Bachelors Degree


Job Description:


We seek a self-motivated individual with some experience in molecular biology and outstanding practical skills to join a small team within the Epigenomics Program at the Broad Institute. The goal of this team is to develop a foundation of knowledge about the state of the Epigenome in normal tissues, and how the Epigenome is altered in disease. As a member of this team, you will produce ChIP-Seq (chromatin immunoprecipitation) libraries, and help improve and develop laboratory methods for ChIP-Seq. You will purify and immunoprecipitate mammalian chromatin, construct libraries, and prepare samples for genome sequencing and other applications. You will optimize reaction conditions, troubleshoot experiments, and contribute to methods development. You will participate in the validation of materials used in our library production process. You will learn to review genome scale data, as part of the quality assurance process. There will be opportunities to learn to automatically sequence DNA using an Illumina HiSeq, to learn to operate an Agilent Bravo for robotic library construction, and to develop pilot collaborations with clinical investigators. In addition, this hire will have the opportunity to develop strong skills in project management and to divide time between laboratory and project management responsibilities. The successful candidate will possess a solid knowledge of molecular biology, and a working knowledge of some of the following: the use of antibodies, immunoprecipitation, the purification, size fractionation, and enzymatic modification of DNA, PCR, and mammalian cell culture. In addition, the successful candidate will interact well with other team members, adapt flexibly to the dynamic challenges of an academic research environment, facilitate project completion, address technical problems, and summarize and present project results.Please see the following URLs for representative recent publications from our extended group: and


* Applies technical knowledge and practical skills in molecular biology to execute laboratory projects that support the overall research goals of the Epigenome Initiative

* Consults with supervisor and other research staff on priorities and methods

* Isolates chromatin from cells or tissues and performs chromatin immunoprecipitation and Illumina library construction

* Carries out laboratory processes, optimizes reaction conditions, analyzes results and addresses technical problems. Presents results to the group, and participates in the design of follow-up experiments

* Coordinates with other research staff to facilitate the completion of pilot studies

* Consults scientific literature and collaborates with other researchers from the scientific community as needed to understand rationale for methods in use, and path to improvement.

* Maintains lab records using a computerized information management system / database, and complies with all safety, biohazard and radioactivity guidelines Requirements:

* Bachelor's degree in Biology or related field required

* One or two post-college years professional experience in Molecular Biology, Genetics, Genomics, or a related field is highly desirable

* Solid technical knowledge and hands-on experience with DNA methods such as purification, analytical and preparative gel electrophoresis, enzymatic manipulation, PCR, sequencing, and molecular cloning required; experience can be from work during undergraduate education.

* Experience in Next-gen library construction, sequence analysis tools and genomics databases is a plus.

* Experience with antibodies and western blotting is a plus. Mammalian tissue culture experience is a plus.

* Strong organizational and time management skills required

* Fluency with Microsoft Excel highly useful for this position

* Must be able to work independently as well as part of a team in a fast-paced environment

* Creativity, scientific curiosity, and the desire and ability to improve existing technology preferred

* Excellent communication skills and the ability to interact professionally with all levels of staff required
EOE / Minorities / Females / Protected Veterans / Disabilities
The Broad Institute will not offer visa sponsorship for this opportunity.