Research Associate I

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POST DATE 9/8/2016
END DATE 11/30/2016

Broad Institute Cambridge, MA

Cambridge, MA
AJE Ref #
Job Classification
Full Time
Job Type
Company Ref #
Entry Level (0 - 2 years)
Bachelors Degree


Job Description:

The Broad Institute of MIT & Harvard, a world leader in genetic research, is looking for exceptional candidates to join the Cardiovascular Disease Initiative (CVDi). The successful candidate will join a multidisciplinary team to assist in the investigation of cardiovascular disease using genetics, cell biology, biochemistry, cell lines and model systems. The current project will focus on understanding proteomic networks perturbed in myocardial infarction under the direction of Drs. Kasper Lage and Sekar Kathiresan. This project is the first of its kind to leverage recent genetic discoveries with large-scale proteomics experiments to derive human vascular proteomic networks for therapy.

The candidate will work closely with Broad CVDi team, computational biologists, and the proteomic platform to perform large-scale pull-down experiments in human vascular cells. The pull-down data will be integrated to understand vascular networks perturbed by genetics in myocardial infarction.

Key Responsibilities:

* Develop, perform and optimize cell-based pull-down assays to generate high-quality protein-interaction samples for mass spectrometry * Maintain cell lines and organize cell bank * Work independently to design and execute research plans to ensure that critical project requirements, timelines and deliverables are met * Troubleshoot scientific problems with minimal guidance * Analyze experimental data and communicate scientific results to team members * Prepare, order, and maintain stocks of necessary reagents and supplies * Attend project meetings to present results and coordinate with other scientists to support project goals


* Bachelor's degree in Biology, Biochemistry, or Chemistry or related field 2+ years experience; or MS in a relevant field with 1+ years of related experience * Familiarity with molecular biology (cloning, PCR, RT-PCR) and biochemistry (protein purification, enzymatic assays, western blot, ELISA) preferred * Strong cell culture experience is required, experience with primary cell transfection preferred * Ability to critically read, interpret, and adapt scientific literature * Strong communication and presentation skills (verbal and written) * Strong organization, documentation and time management skills * Ability to build excellent working relationships and foster collaboration in a team environment

The diverse expertise of the Broad CVDi platform fosters further opportunities for the successful candidate to engage in broad activities, including participation in current industry partnership for target-based drug development.

The Broad Institute will not offer Visa sponsorship for this opportunity

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