Research Associate I - Stanley Center for Psychiatric Research
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The goal of the Stanley Center for Psychiatric Research at the Broad Institute is to improve mental health through research. Over the last several years, the Stanley Center and the psychiatric genetics community have gained unprecedented insight into the genetic basis of schizophrenia, autism and bipolar disorder. Our focus now is to determine how the genetic findings contribute to disease mechanisms, which is essential for the identification of drug targets and eventually the development of effective treatments.
Broadly speaking, the Stem Cell team at the Stanley Center leverages human pluripotent stem cell (hPSC) biology and in vitro differentiation in order to investigate causal relationships between disease-associated genetic variants and neuronal phenotypes. This includes using patient-derived induced pluripotent stem cell (iPSC) lines as well as CRISPR/Cas9-based genome engineering technology, and we are searching for a talented and responsible stem cell biologist to support these efforts. The candidate's background must demonstrate a proven ability to work collaboratively and to contribute to high quality research projects at predetermined milestones.
Perform research and/or development in collaboration with others. Make observations, analyze data and interpret results. Investigate new methods and technologies for project advancement. Contribute to various projects to support the overall research goals. Communicate with all levels of staff and external collaborators to ensure that work supports team goals. Work on problems in which analysis of data requires evaluation of identifiable factors. Exercise judgment within generally defined practices and policies in selecting methods and evaluation criteria for obtaining results. The focus of each experiment and the type of techniques employed may vary in accordance with shifts in team goals.
PRINCIPAL DUTIES AND RESPONSIBILITIES
Under the nominal direction of Dr. Eggan, the candidate will participate in lab projects that include the differentiation of embryonic stem cells and induced pluripotent (iPS) cells to cells of neuronal lineage and to use those cells for addressing biochemical questions. In addition, the candidate will contribute to efforts involving the characterization of the differentiated cells. Specific responsibilities of this position will include:
- Stem cell maintenance and differentiation
- Validation of antibodies against proteins of interest
- Protein quantification, western blotting and immunoprecipitation from cellular material- Molecular and cellular characterization of the cells using molecular and cellular biology techniques including FACS, immunostaining, gene expression
- Performs related laboratory maintenance such as maintaining and cleaning equipment, ordering and keeping an inventory of laboratory supplies
This position requires a strong commitment and the willingness to work irregular hours, but it offers the opportunity to work in a stimulating environment in close proximity to several of the pioneers in the field using state-of-the-art facilities. This is a grant-funded position that is renewable on a year to year basis.
A Bachelor's degree in biology or a related field and mammalian tissue culture experience is required Computer experience is essential.
The ideal candidate will have experience with molecular biological methods and biochemical assays. Comfort with small animal models is a plus. Excellent communication, organizational skills and great attention to detail are essential components of this position. In addition, the ability to excel in a collaborative, team-oriented environment is important. Some weekend flexibility will be required.