Research Technician II

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POST DATE 8/23/2016
END DATE 2/4/2017

Massachusetts General Hospital(MGH) Charlestown, MA

Charlestown, MA
AJE Ref #
Job Classification
Full Time
Job Type
Company Ref #
Entry Level (0 - 2 years)
Bachelors Degree


Our goal at the Genetic and Aging Unit, in the Neurology Department is to identify novel genetic factors that predispose individuals to Alzheimers disease. We have identified numerous pathogenic genetic variants in the past three decades, in addition to replicating findings from other Alzheimer's studies. The knowledge of genetic risk factors is an essential pre-requisite for the development of comprehensive and reliable early-prediction and prevention strategies. The genetics and aging Unit has multiple large and uniformly ascertained Alzheimers disease study samples to aid in the investigation of disease-causing genes. To date, all the genetic findings that increase susceptibility to Alzheimers disease make up for about 50% of the cases and we aim to determine the additional genes that play an important role in the disease onset. In our ongoing effort to discover novel genetic factors in Alzheimers disease, we have recently assembled close to 2,800 whole-genome and more than 10,700 whole-exome datasets in large collections of Alzheimer's study samples. The incumbent will work independently under the supervision of Dr. Rudy Tanzi in prioritizing new Alzheimer's genes, and performing downstream functional experiments with other necessary guidance and supervision provided by the Senior Research Technologist.

Responsibilities include:

* Assisting with follow-up genotyping assays and assists in preparing data for replication analyses to validate the new genes identified in our whole-genome and whole-exome analyses.

* Using advanced high throughput genotyping technology will design, optimize and perform SNP genotyping and/or Sanger sequencing to confirm the presence and prevalence of the new AD genes in the replication study samples.

* Depending on the novel genetic findings coming from the above studies, will also design and perform wet-lab experiments, including gene splicing and expression analysis, and quantitative/qualitative analyses of the transcribed protein employing both in-vitro and in-vivo biochemical assays in collaboration with other researchers in the lab as deemed necessary to test the scientific hypothesis.

* Searching various public online data bases for human DNA sequence context

* Organizing data using Excel spreadsheet, and preparing reports on experimental protocols and results.


* Working knowledge of human genetics research, particularly in the pathogenesis of Alzheimers disease.

* Working knowledge and proficiency in using web-based scientific research portals, such as Pubmed, dbSNP, Ensemble, GTex etc.

* Advanced computer skills including Office suite, routine use of windows and Linux OS based applications. Working knowledge of data analysis and management of large biological databases for research.

* Ability to work independently and as a member of a team

* Good communication skills

* Analytical skills and ability to identify and resolve technical issues

Education: Bachelors Degree

Experience: 1 year of directly related work experience required
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.