Research Technologist - Tissue Engineering
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POST DATE 8/24/2016
END DATE 10/15/2016
Massachusetts General Hospital(MGH)
JOB DESCRIPTIONGENERAL SUMMARY/ OVERVIEW STATEMENT:
The Research Lab Technologist will assist in ongoing studies towards the goal of whole organ bioengineering, part of a study within the MGH Center for Regenerative Medicine, in the Laboratory for Laboratory for Organ Engineering and Regeneration. These studies entail cell culture, expansion, and bio-banking, assistance in preparation of whole organ scaffold by decellularization, and maintaining and monitoring organs during regeneration in the bioreactor. Additional analytical responsibilities include light and fluorescence microscopy, histologic analysis, molecular biology, biochemical assays, and database management.
Under the general supervision of Laboratory Director, Laboratory Manager, and Sr. Research Fellow, the Technologist will carry out a broad range of research activities and procedures with some latitude to determine techniques and to establish protocols. The Technologist will perform lab procedures with speed, precision, and understanding of the theory behind these techniques and assays.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
* Perform isolation, expansion, and banking of primary cell populations from tissues. Utilize experience with cell culture and aseptic technique.
* Perform directed differentiation and expansion of iPSC-derived cell populations by established protocols. Additional responsibilities involve the development of modifications to existing techniques and experimental protocols, or establishment of new procedures.
* Assist in the decellularization of rat, porcine, and human organs. This includes the preparation and changing of sterile solutions in an aseptic manner.
* Assist in the recellularization of large-scale organ scaffolds. Monitor and analyze the regenerating organs during ex vivo biomimetic culture. This includes the collection and analysis of pre-expanded cell populations, organ scaffold preparation and handing, and changing of cell culture media in the bioreactor as needed.
* Complete analysis of cells and tissue by qPCR, ELISA, western blot, and light and fluorescent microscopy.
* Collect and record study data, synthesize and interpret information obtained from graphs, charts, laboratory experiments, statistical summaries and other data collection methods. Information synthesis and interpretation involve use of computer programs as well as data entry into databases.
* Keep detailed laboratory notebooks and records, and prepare written reports on research status, technical procedures, and research protocols. Must maintain a laboratory notebook that includes statistical and graphic summaries of findings and possible conclusions. Collaborate with supervisor in writing and editing material for publication and may appear as principal or secondary author on publications, including organizing and drafting materials for publication and performing independent literature searches.
* Prepare and present informal and formal reports for lab meetings.
* Operate and maintain moderate to complex lab equipment such as autoclave, bioreactors, computer-controlled pumps, qPCR machine, plate reader, light and fluorescent microscopes, and other scientific equipment related to research activity.
* Participate in the upkeep of the laboratory, including direct and general work spaces and help maintain an overall safe and clean environment. May perform, or refer as needed, routine maintenance of lab equipment including cleaning and replacing of parts. Report equipment malfunctions and needs to management.
* Maintain and order supplies, work safely within the lab environment and in performing work duties. Wear personal protective equipment as required. Implement lab safety protocols.
* Provide functional guidance and assistance to lower level technical personnel, orient and train new Research Technicians and Fellows and teach moderately difficult to complex lab techniques to students and research personnel.
* Work well in team-effort situations and independently with minimal supervision.
* A bachelor's degree in biological sciences is required
* Masters Degree preferred
* At least 3 years of practical full-time laboratory experience required.
* Cell culture, immunohistochemistry and molecular biology experience is required.
* Knowledge of developmental biology and organ physiology is preferred
SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:
Understanding of basic to complex scientific laboratory techniques and terminology
Advanced skills and ability to perform laboratory research and use and troubleshoot standard lab equipment
Knowledge of specialized techniques to conduct research projects, including cell culture, aseptic technique and western blot
Must be able to work independently and have demonstrated excellent organizational and excellent oral and written English communication skills.
Able to work carefully, accurately and consistently with instruction or from protocol
Must be able to work safely and properly use protective equipment
Proficiency in math, Word, PowerPoint, Excel, GraphPad Prism, and Internet searches required
Sound interpersonal skills
Ability to identify problems and develop solutions.
Ability to prioritize tasks and meet deadlines.
Function with speed, precision and an understanding of theory behind tests and procedures
Able to work with small animals
Is knowledgeable and compliant in all hospital, State and Federal regulatory requirements, including hospital policy and procedures (where applicable to the performance of the job), Joint Commission on Accreditation of Healthcare Organizations (JCAHO), and Health Insurance Portability and Accountability Act of 1996 (HIPAA).
Duties will be performed in a modern, well equipped research laboratory.
Ability to serve as a support and resource for Research Technicians I and summer interns.
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.