#81628 Laboratory Researcher - Cancer Center
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POST DATE 9/3/2016
END DATE 11/17/2016
University of California - San Diego Medical Centers
San Diego, CA
JOB DESCRIPTIONThis position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment or Special Selection: Apply by 4/26/16 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Eligible ACCES or Special Selection clients should contact their Vocational Rehabilitation Counselor for Special Selection.
Established in 1978, UC San Diego Moores Cancer Center is one of just 41 National Cancer Institute-designated Comprehensive Cancer Centers in the United States, and the only one in the San Diego region. Such centers are prominent among the leading institutions in the nation dedicated to scientific innovation and clinical excellence. This designation reserved for centers with the highest achievements in cancer research, clinical care, education and community contributions makes an enormous difference to our patients. Studies show that outcomes are better at NCI-designated centers.
Independently design, perform and analyze including patient-derived xenograft (PDX), primary tumor growth, tumor cell metastasis, or spontaneous tumor models. Design, perform and analyze experiments to evaluate the roles of targeted proteins in regulation of signal transduction molecules related to tumor growth, adhesion, metastasis, angiogenesis, and drug resistance. Characterizing the novel therapeutics for testing their biologic activity using in vitro and in vivo tumor cell systems. Perform biochemical and molecular pharmacology and pharmacogenetic experiments with cancer drugs in vitro and in vivo including execution of basic and advanced biochemical and molecular biology assays. Make significant innovative contributions pertaining to experimental design and methodology by reviewing published scientific data in refereed journals as well as online using search engines and subject-specific websites to make recommended changes and standards to current protocols and practices. Provide training and assistance to staff scientists, post-doctoral fellows and students.
Strong theoretical and technical knowledge of immunology, molecular biology, cellular biology, cancer stem cell and/or protein chemistry and/or Bachelor's Degree and/or equivalent education/experience.
Extensive experience developing mouse models of cancer and metastasis and angiogenesis. Expertise in aseptic surgical techniques in the capacity of researcher/teacher, to include organ manipulation, tissue implantations, and proper use of anesthesia machines.
Experience conducting pharmacology and pharmacokinetics studies of novel anti-cancer agents in murine models by either intravenous injection or intraperitoneal injection of agents and in primary cell or cancer cell lines in vitro.
Experience and knowledge of flow cytometry analysis of tumor cells and on evaluation of molecular and cell biological function of proteins involved in tumor growth and metastasis including the effect of drugs on these proteins or on tumor cell survival, migration, and invasion.
Experience with culturing primary tumor cells, cancer cell lines and cancer stem cells in vitro and developed expertise in molecular cell biology techniques.
Experience performing molecular and cellular immunology experiments including: DNA isolation, RNA isolation, ELISA assays, immunophenotyping, vector purifications, isolations and transfections, Western blotting, and the construction of vectors and preparation of plasmids and viruses.
Experience using immunophenotyping (cytometry) machines such as FACScan, viral transfection machine and incubators for tissue culture.
Knowledge and experience in training lab personnel and students. Patience, knowledge and experience in order to teach principal laboratory operations, theories, molecular biology techniques, tissue culture operations and techniques to lab members.
* Theoretical and practical knowledge of the Xenogen IVIS 200 and OV100 live-imaging system
* Must be willing to work occasional evenings or weekends as required. Must be willing and able to work with small animals and biohazardous materials.