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Staff Research Associate III
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University of California - Los Angeles Medical Centers University of California - Los Angeles Medical Centers
Los Angeles, CA
- Job Type
- Job Classification
Mid-Career (2 - 15 years)
- Company Ref #
- AJE Ref #
Staff Research Associate III
Staff Research Assoc III
9-954 Factor Building - Westwood, CA
$23.45 / $4080
$37.70 / $6560
Layoff Referral Deadline:
Primary duty will be to provide technical support in a research laboratory. Planning, organizing, conduction and analyzing basic research. Perform various techniques involving tissue culture of mammalian cells with sterile techniques, DNA/RNA/protein extraction/quantification from biological materials of different types, qRT-PCR, Western Blot, work in a mouse colony. The candidate should be self-motivated, show initiative and be able to work independently on projects including filling records meticulously, maintaining laboratory equipment and supplies, stocking and placing laboratory orders. Strong organizational skills, attentiveness to details, multi-tasking, strong communication skills and troubleshooting abilities are a must. Must be able to work independently.
BS degree in Life Science or other related discipline preferred. Working knowledge and experience in general immunology, cell biology and molecular biology techniques. 5 + years experience in cell and tissue culture. Experience working in GMP regulated environment. Excellent record-keeping skills. Excellent communication skills both verbal and written. Excellent organizational and multitasking abilities. Ability to complete tasks with general instructions and follow up on problems. Flexibility of working hours to align with the manufacturing schedule. Prior experience with tumor immunotherapy, including adoptive cell transfer with TCR transgenic lymphocytes, carcinogen-induced skin cancers and immune-modulating antibody therapies. Knowledge of viral vector production and transduction of mammalian cells. Working knowledge of patient sample processing (tissue, blood). Ability to use computer software for word processing, data tabulation and graphing. Knowledge and ability to order supplies, maintain laboratory equipment and write SOPs. Ability and knowledge to operate basic laboratory equipment, i.e. centrifuge, tissue culture hood and related equipment, autoclaving, weighing, pipetting microliter volumes etc. Ability and working knowledge in cell proliferation and cytotoxicity assays, measurement of cytokines by Elisa and multiplexing, immunohistochemistry, Endotoxin and Mycoplasma assays. Ability and working knowledge of Molecular Biology techniques including DNA, RNA, protein purification and quantification; PCR, qPCR, Western Blot. Prior experience in Flow Cytometry technique. Prior experience in Fluorescent and Confocal microscopy. Ability to train laboratory staff.