Staff Research Associate - Westwood
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POST DATE 8/12/2016
END DATE 10/11/2016
University of California - Los Angeles Medical Centers
Los Angeles, CA
JOB DESCRIPTIONAPPLY Job Title:
Staff Research Associate - Westwood
Staff Res Assoc II-Nonexempt
Monday - Friday, 8:00am - 5:00pm
$21.27 / $3701
$34.23 / $5956
Layoff Referral Deadline:
Maintain the animals in the UCLA Humanized Mouse Core Facility in BSRB. Inspect animals and monitor mouse health daily and record observations into a database. Treat mice daily and document treatments into a database. Weigh mice weekly and record measurements into a database. Perform animal manipulations independently injections (retro-orbital, intra-peritoneal, subcutaneous), retro-orbital bleeding, euthanasia, tissue harvesting, biopsies and record detailed observations into a database. Assist with mouse humanization surgeries prepping mice, performing surgeries, post-surgery treatment. Perform tissue processing of human liver and thymus. Perform PCR and gel electrophoresis for tissue sub-typing. Perform mouse tissue processing (blood, spleen, implant, liver, lung, brain, bone marrow). Perform tissue culture work with primary cells, cell staining, flow cytometry. Perform DNA and RNA extraction, PCR and RT-PCR. Perform computer and paperwork. Maintain laboratory notebook in enough detail that results can be reproduced. Independently Order, stock, and maintain inventory of Core supplies. Provide written status of weekly activities. Proactively notify supervisor of any anomalies or irregularities in mouse population, or Core operations. Special projects as requested.
Must have taken biology classes. Working knowledge and experience with basic biology. Skill in reading scientific papers and applying relevant information. Able to work several hours in Tyvek/surgical gown/pants, mask and gloves. Willing to work long hours (planned or unplanned), overtime, or alternate hours (such as weekends, offset hours and late at night) as requested. Skill in performing a given protocol in a reproducible way. Skill in reading laboratory protocols and other scientific documents. Skill in troubleshooting and optimizing protocols as necessary, documenting any protocol deviations with thorough explanation of why deviation occurred. Ability to work independently to finish tasks in a timely manner. Ability to set priorities and manage time effectively despite unanticipated frequent interruptions/distractions. Ability to adjust to changes in the workload and switch focus as needed. Ability to make sound judgments and decisions. Very organized with meticulous attention to detail. Skill in detailed documentation of activities, tasks, progress, observations, etc. Ability to troubleshoot laboratory equipment malfunctions and identify root cause. Skill in using Microsoft Word, Excel, PowerPoint, Google Docs, Dropbox, Mac computers, iPad. Familiarity with FlowJo. Skill in maintaining accurate and detailed records of experiments and paperwork. Skill in creating and maintaining computerized records of experiments and data; skill in identifying anomalies in data or results, and proactively notifying supervisor in a timely manner. Skill in maintaining records of supplies and ordering supplies; proactively maintaining appropriate inventories. Skill in researching new supplies i.e. cost effective methods and new technologies. Skill in performing mathematical calculations, such as dilution factors, titrations, accurately and without error. Skill in using common laboratory equipment, including: micropipettes, pipets, microscopes, centrifuges, ultracentrifuges, CO2 incubators, water baths, electrophoresis equipment, thermal cyclers, flow cytometers, autoclave. Skill in using BSL2/BSL2+ laboratory practices and sterile techniques. Ability to work with HIV in a biosafety cabinet. Ability to grow virus stocks and submit for p24 assays. Skill to safely handle HIV to infect humanized mice. Skill in safely handling HIV infected mice. Skill in performing animal manipulations independently various injections, retro orbital bleeding, euthanizations, tissue harvesting. Skill in processing mouse tissues (blood, spleen, thymus, lung, liver, brain, bone marrow, and more) in a biosafety cabinet. Skill in managing breeding of mice analyze breeding patterns to determine when to set up and cull breeding pairs. Ability to analyze usage of mice to determine appropriate numbers of mice to breed, house in breeding rooms, and house in research rooms; determine when to submit transfers. Skill in documenting breeding data and proactively informing supervisor of any anomalies. Skill in maintaining computer records to keep track of mouse health. Skill in analyzing weights, treatments, and various other metrics to determine when to treat animals or set endpoints. Skill in documenting observations and providing summary data to supervisor; proactively informing supervisor of any anomalies. Skill in analyzing metrics to determine when to proceed to later stages of an experiment. Skill in documenting observations and providing summary data to supervisor; proactively informing supervisor of any anomalies. Ability to analyze and monitor human cell engraftment in humanized mice; proficiency in entering data into database and documenting data analysis to supervisor. Ability to help users develop animal based studies; e.g., recommending protocols, optimum sample size, relevant metrics, etc. Ability to process human liver and thymus. Ability to perform HLA-subtyping; analyze results based on a standard kit; Skill in preparing media and reagents used in tissue culture work. Skill in handling human cells and cell lines (adherent and suspension). Skill in counting cells, freezing/thawing cells, storing cells, passaging cell lines, maintaining primary cells. Skill in documenting observations and providing summary data to supervisor; proactively informing supervisor of any anomalies. Skill in isolating cell sub-populations from human and mouse blood/tissue. Ability to perform lentiviral vector transfection, transduction, and titration. Skill in flow cytometry staining of samples (cells, blood, tissue). Familiarity with flow cytometry acquisition and analysis; ability to learn MACSQuant flow cytometer and become proficient in operation in a timely manner. Skill in performing DNA/RNA extractions, PCR, RT-PCR, gel electrophoresis. Skill in database management, utilizing inventory software.