TECHNICAL RESEARCH ASSISTANT II / 40 HOURS / DAY / BWH - PULMONARY 9/14/2016
Brigham & Women's Hospital(BWH)
JOB DESCRIPTIONAPPLY GENERAL SUMMARY/OVERVIEW STATEMENT:
Under minimal supervision, and within established departmental and hospital policies and procedures, the research assistant will be responsible for performing a range of experiments to investigate pulmonary vascular function. Experiments will involve cultured cells and rodent models. Responsibilities may include maintaining cell culture systems and rodent colonies, generating models of pulmonary hypertension, physiologic characterization of rodents, preparation of samples from cultured cells and animals, and analysis of these samples using a variety of approaches. Will assist in the design and development of new assays and research protocols. Responsibilities include overseeing laboratory safety according to established HMS guidelines. Within budget constraints, will order supplies and initiate equipment purchases. May participate in training other lab personnel, although not in a primary supervisory capacity. Will prepare reports and present data to other laboratory members in lab meetings. May also provide support to other related projects when needed.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
1. Maintaining cultured cells, performing treatments (including gene silencing or overexpression using siRNA or viruses), and sample preparation.
2. Analyzing RNA, DNA, and protein samples from animal tissue or cells (including RT-qPCR, Western blots, enzyme assays).
3. Assisting in the design and modification of assays and research protocols, including those involving rodent models.
4. Orienting and training new research fellows and lab assistants in lab procedures.
5. Maintaining accurate records of all experiments, compiling data and performing statistical analyses on results as required.
6. Initiating purchases of new supplies and equipment and maintaining adequate supplies in lab.
7. Maintaining laboratory safety records as necessary. Coordinating laboratory compliance with HMS safety guidelines in cooperation with the principal investigator, lab manager, and HMS safety office.
8. Maintaining a clean and orderly work area, contributing to routine lab maintenance and cleaning.
9. Other duties as assigned by the principal investigator.
1. Bachelor's degree in biological / physical sciences.
2. Minimum one-year laboratory experience in a biomedical research laboratory.
3. Experience with one or more of the following: cell culture, protein analysis, or molecular biology.
4. Experience working with small animals (rats and mice).
2. Organized, meticulous, and with careful attention to detail.
3. Excellent technical skills (pipetting, operating equipment).
4. Self-directed learning and able to work independently.
5. Good oral and written communication skills.
6. High degree of computer literacy (proficiency with Microsoft Office, including Excel and Powerpoint).
7. Analytic ability to resolve technical problems and interpret acceptability of data results.
8. Comfortable working with animals.
Brigham and Womens Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, creed, sex, sexual orientation, gender identity, national origin, ancestry, age, veteran status, disability unrelated to job requirements, genetic information, military service, or other protected status.
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