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POST DATE 8/27/2016
END DATE 4/18/2017

Brigham & Women's Hospital(BWH) Boston, MA

Boston, MA
AJE Ref #
Job Classification
Full Time
Job Type
Company Ref #
Entry Level (0 - 2 years)
Bachelors Degree



Laboratory at the Transplant Research Center seeks a Technical Research Assistant II (TRA-II). The focus of the laboratory is to understand immune regulation in transplantation, using human clinical materials (e.g. peripheral blood mononuclear cells) as well as murine transplant models. Under the direct supervision of Principal Investigator(s), TRA-II will assist with ordering equipment and supplies, managing mouse colonies and processing of biological samples. These studies involve working with animals, preparation of immune cells from mouse tissues or human clinical samples for flow cytometric analysis, and other basic molecular and cell biology techniques, including cell culture and genotyping, and interpretation of flow cytometric data.


1.Work independently under minimal supervision, conduct a variety of general cellular immunology techniques, including: primary cell cultures and culture of cell lines, isolations of various immune cells from mouse and human tissues- functional in vitro cell culture assays- ELISA for cytokine determinations- and flow cytometric analysis of immune cell populations.
2. Maintain breeding colonies of mice, including genotyping, administration of test substances such as antibodies, cytokines, or immune stimulators to mice, and collect data on transplanted animals.
3.Perform organ harvests from animals and process organs and tissues for histological, microbiological and immunological analysis.
4.Prepare data for presentation using scientific graph software and report progress of work at weekly laboratory seminars.
5.Assist in the basic design and modification of protocols.
6.Prepare laboratory stock reagents, chemicals, instruments and equipment.
7.Order equipment and supplies inventory in accordance with laboratory and department procedures.
8.Maintain established department policies, procedures and objectives, including the areas of quality assurance, safety, environmental and infection control.
9.Applicant will be trained to perform any technique not currently familiar with, including handling of laboratory animals. After sufficient training, the technician is expected to work independently with minimal supervision.


Bachelor of Science degree in a biological/physical science or equivalent is required. Minimum of one (1) year experience/post grad experience in a research lab environment studying immunology, molecular biology, microbiology or biochemistry is required. Willingness to work with laboratory animals is preferable.


Well organized, dependable and ability to work independently or as a team member to accomplish research goals. Ability to learn new and innovative procedures quickly. Excellent analytical skills and excellent oral and written communication skills with ability to organize, document, and present research data. Demonstrated ability to use computer software for data generation, statistical analysis and record keeping such as MS Word, MS Excel, GraphPad Prism, FlowJo. On own initiative, ability to identify potential problems and trouble-shoot problems as necessary.


Normal laboratory environment. Principal investigators Lab contains approximately 4 workers, including post-doctoral fellows, students and technicians administrative staff. Exposure to human and animal blood products. Moderate exposure to laboratory mice.


Assists in coordinating and organizing assigned laboratory activities.

Brigham and Womens Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race,
color, religion, creed, sex, sexual orientation, gender identity, national origin, ancestry, age, veteran status, disability unrelated to job requirements, genetic information, military service, or other protected status.

Brigham and Womens Hospital I CARE Standards

The I CARE Standards guide me in my interactions with patients, family members, visitors, colleagues, and neighbors in my workplace and surrounding community to optimize the Brigham experience and help fulfill my role in our mission of providing seamless, high quality patient and family centered care.
C.I will Communicate my commitment to high quality performance
A.I will Appear and act as a professional
R.I will Respect all individuals
E.I will Extend myself