Research Specialist 5/4/2020
Massachusetts General Hospital(MGH)
JOB DESCRIPTIONAPPLY Unique opportunity to join an interdisciplinary team bridging the Harvard Medical School, the Massachusetts General Hospital, and the Broad Institute of Harvard and MIT. The LAWRENCE LAB and HAAS LAB at the Massachusetts General Hospital Cancer Center seeks well-qualified candidates to join a cross-disciplinary team of biochemists, biologists, and clinicians working at the forefront of cancer research and treatment. We use biochemistry, mass spectrometry, and statistical data analysis to study both the fundamental biology of cancer initiation and progression, as well the diagnosis and treatment of cancer patients in the hospital setting.
Current research interests of the groups:
- CANCER EVOLUTION: Cancer is a process of Darwinian evolution occurring inside the patient's body, with an ongoing competition between cells to proliferate aggressively. Changing pressures on the cells, from treatment with chemotherapy or targeted agents, changes the dynamics of this evolution and can lead to one group (clone) of cells displacing another. We use genomic profiling to observe the processes of clonal evolution during the course of a patient's disease and treatment.
- RESISTANCE TO TARGETED THERAPIES: Single drugs targeting specific driver mutations can be effective for a while, but the cancer invariably discovers a way to evolve resistance to the drug. We are actively investigating mechanisms of drug resistance and how to combat it.
- SINGLE-CELL SEQUENCING: New approaches allow us to dissect a tumor down to single cells and investigate the RNA expression or DNA mutations in each cell. Understanding intra-tumoral heterogeneity is shedding new light on cancer progression and patient outcomes.
- LIQUID BIOPSIES: novel state-of-the-art technologies are starting to allow us to monitor the progression of cancer (both before, during, and after treatment) through a simple blood draw. We are actively working to overcome analytical challenges inherent in the study of circulating tumor cells (CTCs) and cell-free circulating tumor DNA (ctDNA).
- MUTATIONAL PROCESSES: Our genomes accumulate mutations from environmental agents such as ultraviolet radiation and tobacco smoke, as well as from intrinsic processes like errors during DNA replication. Studying these mutational background patterns can tell us what repair pathways are broken in a specific tumor, perhaps pointing the way to an effective genotoxic therapy. We are working to develop novel DNA sequencing technologies for studying mutagenesis in model systems.
- CANCER DRIVER GENES: Tumors grow because of specific driver mutations that deactivate tumor suppressors or activate oncogenes. We are working to complete our understanding of the full catalog of cancer's "box of tricks".
As a Biochemist, you will use state-of-the-art technologies to assist in sample preparation, testing, and analysis, using biochemistry and mass spectrometry, followed by statistical data analysis. You will contribute to writing reports and creating visualizations of biochemistry experimental results and communicate our findings to our collaborators in other MGH laboratories and clinical groups.
* Possess an understanding of laboratory procedures and under general supervision can conduct complex analysis.
* Perform a variety of routine sample preparation and analysis procedures to quantitatively measure pharmaceutical and biopharmaceutical compounds in a variety of formulations and/or biological matrices for stability and analytical testing.
* Train on routine operation, maintenance and theory of analytical instrumentation, SOPs and regulatory guidelines.
* Responsible for review and compilation of results and data comparison against SOP acceptance criteria, methodology, protocol and product specifications.
B.A./B.S. (M.A./M.S. preferred) in Biochemistry.
At least 7years of experience in scientific research.
Experience in PCR, protein purification, spectroscopy, and chromatography.
Independent, self-motivated drive to push research forward.
Knowledge of routine operation, maintenance and theory of analytical instrumentation, SOPs and regulatory guidelines.
Maintain a laboratory notebook and complete all laboratory documentation in clear and accurate language and according to SOP.
Manage time effectively to complete assignments in expected time frame.
Comfortable using Word, Excel, PowerPoint to communicate results between team members.
Ability to work together with multi-disciplinary teams comprising physicians, biologists, statisticians, and software engineers.
Fluency in spoken and written English.
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.