Research Technician 6/3/2020
Massachusetts General Hospital(MGH)
JOB DESCRIPTIONAPPLY We are seeking a bright and motivated individual for a Research Technician I position in the laboratory of Sarita Patil, M.D. in the Center for Immunology and Inflammatory Diseases (CIID). The CIID is a multidisciplinary research center whose goal is to discover new mechanisms and molecular pathways in allergic, autoimmune and inflammatory diseases.
The main interest of the Patil lab is understanding the role of antigen-specific B cells and humoral immunity in allergic diseases. Our lab is mainly focused on human immunology, utilizing peripheral blood and human tissues from allergic subjects enrolled in various clinical trials, as well as non-allergic blood donors.
Our systems-based approach to study various aspects of the humoral system in depth involves a variety of research techniques, including the development of antigen-specific B cell tetramers, single-cell and bulk cell isolation, recombinant antibody production and isolation, cell culture, PCR, flow cytometry and cell sorting, next generation sequencing (RNAseq, BCRseq), protein mass spec, antibody characterization using biolayer inferometry, and effector cell assays. In addition, our laboratory performs our own bioinformatics analysis, using several software and programming platforms for data collection and analysis. Our lab is highly collaborative, and members are engaged in a variety of projects across different institutions. We are looking for a research technician to play an interactive and integral role in these studies.
Candidates for this position should have a solid background in biology and a strong interest in immunology and molecular biology. In addition, experience with computer programming and complex data analysis would be a significant asset. This position would be ideal for those candidates interested in gaining significant experience in biomedical research and considering future graduate studies.
DUTIES WILL INCLUDE:
* Molecular biological techniques, including DNA and RNA isolation, PCR, and antibody production and isolation
Isolation, purification, culturing and cryopreservation of primary white blood cells from human samples
Flow cytometry and cell sorting, including single-cell sorting
Participation in general laboratory responsibilities, including ordering and organizing laboratory supplies and reagents, safety maintenance, and laboratory equipment maintenance
Administration and management of patient sample repositories
Participation in collaborations
Aiding in complex data analysis of large datasets
* Bachelors Degree, with coursework in related fields such as Immunology, Molecular Biology and/or Biochemistry
* Previous laboratory experience is strongly preferred, even though required techniques will be taught upon joining the lab
* Proficiency with computer software, including Word, Power Point and Excel
* Experience with the programming language R or other programming languages would be an asset
* Must be reliable, organized, and able to work independently as well as with other members of the team and collaborators
* Excellent communication and interpersonal skills
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.